The NC3Rs hosted a webinar on Data‑Driven Design of Fish Cell Line‑Based Reproductive Toxicity Assays on 9 December 2025, from 14:00 to 15:00 CET. This free, one‑hour online session presented key outcomes from the SAFE CRACK IT Challenge, a collaborative initiative led by aQuaTox Solutions GmbH, Eawag, and the National Institute of Biology (Slovenia), aimed at advancing New Approach Methodologies (NAMs) to reduce reliance on in vivo fish testing for environmental risk assessment. Participants learned about a novel computational pipeline for identifying reproductive toxicity biomarkers, expression data generated from rainbow trout liver and testis cell lines, and machine‑learning models built on historical and newly generated in vitro and in vivo datasets.

Target audience
This webinar was particularly suited for ecotoxicologists and regulatory scientists from industry, academia, and government agencies involved in the development, evaluation, or regulatory acceptance of NAMs.